ABEL® Oxidative Stress Kits with Pholasin®:
- Cell Activation Test Kits
- Antioxidant Test Kits
Knight Scientific Limited (KSL) produces a range of unique oxidative stress test kits for measuring free radicals and other non-radical reactive oxygen containing species and for determining the antioxidant potency of raw materials, ingredients and finished products. The highly reproducible relative antioxidant capacity scores (ABEL-RAC™) can be used on labels of finished products, in formation development, quality control and quality assurance.
KSL also offers a confidential contract testing service. The choice is yours: contract testing for you or in-house testing by you, the choice is yours.
Please contact KSL to discuss your requirements or for technical information and advice.
ABEL® Cell Activation Kits with PHOLASIN®
Measure the real time production of free radicals and other non-radical reactive oxygen species (ROS) during the respiratory burst, from pieces of tissue, materials, enzymes and drugs and can also monitor degranulation of white blood cells.
They have many diverse applications including:
- high performance sport
- exercise physiology
- inflammatory disease management
- stress management
- drug evaluation
- vaccine testing
- biocompatibility testing
- materials testing
- wound healing
- skin research
KSL has many years experience of research involving oxidative stress and would be very pleased to help you decide on the most appropriate kit and experimental design.
| Product | Cat No. | Size |
|---|---|---|
| Cell Activation Test Kits for Whole Blood with Pholasin® & Adjuvant-K™ (tube/cuvette format) |
ABEL-04 | 40 × 1mL |
| ABEL-05 | 80 × 1mL | |
| ABEL-06 | 120 × 1mL | |
| Cell Activation Test Kits for Whole Blood with Pholasin® & Adjuvant-K™ (microplate format) |
ABEL-00M | 100 × 200µL |
| ABEL-04M | 200 × 200µL | |
| ABEL-05M | 400 × 200µL | |
| Cell Activation Test Kits for Isolated Cells with Pholasin® & Adjuvant-K™ (tube/cuvette format) |
ABEL-14 | 40 × 1mL |
| ABEL-15 | 80 × 1mL | |
| ABEL-16 | 120 × 1mL | |
| Cell Activation Test Kits for isolated cells with Pholasin® & Adjuvant-K™ (microplate format) |
ABEL-10M | 100 × 200µL |
| ABEL-14M | 200 × 200µL | |
| ABEL-16M | 400 × 200µL |
ABEL® ANTIOXIDANT TEST KITS WITH PHOLASIN®
- Quantify antioxidant activity
- A range of different antioxidant tests
- ABEL-RAC™ (Relative Antioxidant Capacity)
- ABEL-RAC™ scores on finished products
- ABEL-RAC™ scores per mg or µL sample
- ABEL-RAC™ scores per dose
- Test raw materials, ingredients & finished products
- Identify possible synergy between ingredients
- Optimise formulations
- Monitor processes during manufacture
- Confirm batch to batch uniformity
- Quantify shelf life of ingredients and products
These chemiluminescent test kits contain the unique photoprotein Pholasin® which emits light in the presence of free radicals and certain oxidants. The antioxidant capacity of the sample under test is expressed as the percentage reduction of peak luminescence of Pholasin® (and/or the delay in time in which the peak luminescence is observed) during the course of the test in the presence and absence of the sample. The results can be expressed against other antioxidant standards or as ABEL-RAC™ (relatively antioxidant capacity) scores per mg or material being tested.
Samples of serum or plasma of people and other animals are expressed as TAC (total antioxidant capacity) scores against a set of antioxidant standards of known concentration. These ABEL-TAC™ scores have known distribution ranges.
It is now possible to measure the antioxidant activity in a sample of blood taken from a mere finger prick to determine the TAC of individuals and classify their scores. These precise measurements make people aware of their antioxidant status, providing reassurance or enabling them to take steps to remedy a low value.
| Product | Cat No. | Size |
|---|---|---|
| Antioxidant – Superoxide & Other Free Radicals with Special Pholasin® (tube/cuvette format) |
ABEL-21 | 25 × 1mL |
| ABEL-22 | 50 × 1mL | |
| ABEL-23 | 100 × 1mL | |
| ABEL-24 | 500 × 1mL | |
| Antioxidant – Superoxide & Other Free Radicals with Special Pholasin® (microplate format) |
ABEL-21M2 | 100 × 200µL |
| Antioxidant – Halogenated Oxidants with Special Pholasin® (tube/cuvette format) |
ABEL-31 | 25 × 1mL |
| ABEL-32 | 50 × 1mL | |
| ABEL-33 | 100 × 1mL | |
| ABEL-34 | 500 × 1mL | |
| Antioxidant – Halogenated Oxidants with Special Pholasin® & (microplate format) |
ABEL-31M | 100 × 200µL |
| Antioxidant – Total Antioxidant Capacity (TAC) test the wide range antioxidant test with Peroxynitrite & Pholasin® (microplate) |
ABEL-40M2 | 100 x 200µL |
| ABEL-41M2 | 200 × 200µL | |
| Antioxidant – Hydroxyl radical with special Pholasin® (tube/cuvette format) |
ABEL-53 | 100 × 1Ml |
| Antioxidant – Hydroxyl radical with special Pholasin® (microplate format) |
ABEL-51M | 100 × 200µL |
| Product | Cat No. | Size |
|---|---|---|
| Superoxide and SOD quantification kit with Pholasin®, xanthine & xanthine oxidase | ABEL-60M | 100 × 200µL |
| Superoxide detection kit with Xanthine Oxidase 200 test kit | ABEL 61M | 100 × 200µL |
ABEL® Antioxidant Test Kits for Tube and Microplate Luminometers
- ABEL® 21 series:
Antioxidant Test for superoxide and other free radicals is excellent at screening for antioxidants such as ascorbic acid.
- ABEL® 31 series:
Antioxidant Test for halogenated oxidants such as hypochlorous acid, produced naturally by some white blood cells, assesses antioxidants such as albumin. It is excellent for testing materials that are dissolved in organic solvents.
- ABEL® 41 series:
Antioxidant Test using peroxynitrite is a total antioxidant capacity (TAC) test because peroxynitrite attacks virtually any substance that has antioxidant properties and can screen for both water and lipid soluble antioxidants.
- ABEL® 51 series:
Antioxidant Test for hydroxyl radical is an excellent assay for assessing antioxidants in a variety of materials including plants.
- ABEL® 61 series:
Antioxidant Test with enzyme generated superoxide produced in the xanthine/xanthine oxidase reaction, excellent for quantifying superoxide and for measuring the activity of superoxide dismutase (SOD) and SOD mimetics. It is possible with this assay to distinguish between quenchers of superoxide such as Vitamin C and SOD mimetics.
