ABEL® Antioxidant Test Kit for Peroxynitrite with Special Pholasin®
ABEL® Antioxidant Test Kit for Peroxynitrite with Special Pholasin®
Additional Information
The Pholasin® emits light in the presence of peroxynitrite formed continually by the reaction between superoxide and nitric oxide released from SIN-1. In the absence of antioxidants a peak of light with Pholasin® occurs at a particular time. Antioxidants in the sample will compete with Pholasin® for peroxynitrite, thereby delaying the TIME at which the peak emission occurs. The antioxidant capacity of the sample tested is expressed in Vitamin E analogue (VEA) equivalent units derived from the peaktimes of a set ot VEA standards. A full plate of 96 wells (5 standards and 44 samples in duplicate) can be assayed in about 30 minutes. Aditionally to anti-oxidan product testing, the results can be compared between individuals as well as used to monitor changes in individuals in response to diet.
Product Specific Literature References:
The ABEL® peroxynitrite antioxidant test with Pholasin® measures the antioxidant capacity of plasma to protect against peroxyl radical attack: J. Knight, et al.; Luminescence 17, 95 (2002)
KSL-ABEL-40M2
Kit/Set Contains:
Wide-Range Antioxidant Test Kit using Peroxynitrite with Special Pholasin® (Microplate Format)
Kit components sufficient for 100 x 200µL tests.
A. 1 x bottle Pholasin® (50µg: reconstitute to 5 ml). Store at -20º.
B. 1 x 25ml Reconstitution & Assay Buffer for Peroxynitrite assay. Store at -20º.
C. 2 x 12mg SIN-1 (mixed with an inert material) (reconstitute to 6ml). Discard any remaining.
D. 1 x 25ml Water for dissolving SIN-1. Store at -20º.
E. 1 x Vit E Analogue Standard: VEA Standard (reconstitute to 2ml). Store at -20º; when reconstituted store at 2-8º.
F. 1 x bottles with 9ml R&A Buffer for VEA standard dilution. Store at -20º.
G. 1 x 96 well white microplates
KSL-ABEL-41M3
Kit/Set Contains:
Wide-Range Antioxidant Test Kit using Peroxynitrite with Pholasin® (Microplate Format)
Kit components sufficient for 200 x 200µL tests.
A. 1 x bottle Pholasin® (100µg: reconstitute to 10 ml). Store at -20º.
B. 1 x 50ml Reconstitution & Assay Buffer for Peroxynitrite assay. Store at -20º.
C. 3 x 12mg SIN-1 (mixed with an inert material) (reconstitute to 6ml). Discard any remaining.
D. 1 x 25ml Water for dissolving SIN-1. Store at -20º.
E. 2 x Vit E Analogue Standard: VEA Standard (reconstitute to 2ml). Store at -20º; when reconstituted store at 2-8º.
F. 2 x bottles with 4.5ml R&A Buffer for high range VEA standard dilution. Store at -20º.
G. 2 x bottles with 4.5ml R&A Buffer for low range VEA standard dilution. Store at -20º.
H. 2 x 96 well white microplates
KSL-ABEL-41M7
Kit/Set Contains:
Wide-Range Antioxidant Test Kit using Peroxynitrite with Pholasin® (Microplate Format)
Kit components sufficient for 200 x 200µL tests.
A. 2 x bottles Pholasin® (50µg: reconstitute to 5 ml). Store at -20º.
B. 2 x 25ml Reconstitution & Assay Buffer for Peroxynitrite assay. Store at -20º.
C. 4 x 12mg SIN-1 (mixed with an inert material). Discard any remaining.
D. 2 x 25ml Water for dissolving SIN-1. Store at -20º.
E. 2 x Vit E Analogue Standard: VEA Standard (reconstitute to 2ml). Store at -20º; when reconstituted store at 2-8º.
F. 2 x bottles with 4.5ml R&A Buffer for VEA standard dilution. Store at -20º.
G. 2 x 96 well white microplates
